Full pixel crop
Tokina 300mm f/2.8 + approx. 50mm extension tube using a modified lensbaby, the aperture ring is set at f/7 1/160 second ISO 3200 in diffused window light on a Nikon D200 hand held at closest focus distance of 4 feet from the sensor, the front element of the lens is 1 foot from the sensor: the lens shade is another 6 inches deep.
The first shot is with a 50mm extension tube, the second with a +2 diopter closeup lens. The closeup lens lets me meter but the working distance is too close for skittish insects and even though I could stop down a little further, the depth of field is very shallow and magnification greater. The actual f/stop would calculate out much larger than what the aperture ring says with an extension tube and I used a -5 exposure compensation to meter.